PMID- 34918502 OWN - Quintessenz Verlags-GmbH CI - Copyright Quintessenz Verlags-GmbH OCI - Copyright Quintessenz Verlags-GmbH TA - Oral Health Prev Dent JT - Oral Health and Preventive Dentistry IS - 1757-9996 (Electronic) IP - 1 VI - 19 PST - epublish DP - 2021 PG - 683-688 LA - en TI - Kgp DNA Vaccine Prevents Experimental Periodontitis LID - 10.3290/j.ohpd.b2448589 [doi] FAU - Jiang, Xiao AU - Jiang X FAU - Li, Chuanhua AU - Li C FAU - Fan, Xin AU - Fan X FAU - Chen, Xu AU - Chen X FAU - Guo, Meihua AU - Guo M FAU - Lan, Jing AU - Lan J CN - OT - DNA vaccine OT - experimental periodontitis OT - IgG OT - Kgp AB - Purpose: To investigate the prophylactic effect of lysine-specific protease (Kgp) vaccine on experimental periodontitis in mice. Materials and Methods: We constructed the eukaryotic expression plasmid pVAX1-kgp and immunised mice with the recombinant plasmid. Mice were divided into two groups and immunised with pVAX1-kgp or pVAX1 three times at 2-week intervals. Immunoglobulin (Ig)G, IgG1 and IgG2a antibodies were detected by enzyme-linked immunosorbent assay (ELISA) before and after immunisation. At the last immunisation, a silk ligature infiltrated with Porphyromonas gingivalis (P. gingivalis) was tied at the neck of the maxillary second molar to induce experimental periodontitis. Each group was euthanised after 10 days, and microcomputed tomography (micro-CT) and hematoxylin-eosin (HE) staining were used to detect the loss of alveolar bone. Results: Comparison with the pVAX1 group indicated that mice immunised with Kgp had higher levels of IgG (P < 0.05); the levels of the IgG1 were statistically significantly different (p < 0.05), and the levels of the IgG2a subtype were not significantly different. The results of micro-CT and HE staining showed that the alveolar bone loss in the pVAX1-kgp group was statistically significantly less than that in the pVAX1 group (p < 0.05). The expression of the related inflammatory factors, including interleukin-1β (IL-β), tumour necrosis factor (TNF-α) and interleukin-6 (IL-6), was lower in the pVAX1-kgp group than in the pVAX1 group. Conclusion: The Kgp DNA vaccine can enhance IgG levels in a model of experimental periodontitis, effectively activate immunity, and mitigate alveolar bone loss. AID - 2448589