OWN - Quintessenz Verlags-GmbH CI - Copyright Quintessenz Verlags-GmbH OCI - Copyright Quintessenz Verlags-GmbH TA - Quintessence Int JT - Quintessence International IS - 1936-7163 (Electronic) IP - 10 VI - 36 PST - ppublish DP - 2005 PG - 812-816 LA - en TI - Antimicrobial spray for toothbrush disinfection: An in vivo evaluation FAU - Sato, Sandra AU - Sato S FAU - Pedrazzi, Vinícius AU - Pedrazzi V FAU - Guimaraes Lara, Elza Helena AU - Guimaraes Lara E FAU - Panzeri, Heitor AU - Panzeri H FAU - Ferreira de Albuquerque jr., Rubens AU - Ferreira de Albuquerque jr. R FAU - Ito, Izabel Yoko AU - Ito I CN - OT - antimicrobial agent OT - bacterial contamination OT - biofilm OT - chlorhexidine OT - disinfection OT - oral hygiene aids OT - spray OT - toothbrush AB - Objective: The aim of this study was to evaluate the efficacy of a spray containing an antimicrobial solution for toothbrush disinfection. Methods and Materials: Three different solutions were sprayed on toothbrush bristles among 30 adults after they had brushed: (1) basic formulation (base) plus chlorhexidine; (2) base only, and (3) sterile tap water (control). Each solution was tested for 1 week. After that, the toothbrushes were collected and sonicated in Letheen Broth, diluted in 10-fold series, and plated on selective and nonselective media for detection of anaerobes, aerobes, streptococci, and gram-negative bacilli. After incubation, the colonies of those microorganisms were counted. Presence of mutans streptococci on the bristles was also confirmed. Results: Spray 1 produced a significant reduction in the microbial contamination of toothbrushes for all the microorganisms, spray 2 provided some reduction of contaminants, and spray 3 demonstrated the least antimicrobial effect. Conclusion: The antimicrobial spray with chlorhexidine proved to be an effective and practical means for toothbrush disinfection. AID - 839620