PMID- 26295068 OWN - Quintessenz Verlags-GmbH CI - Copyright Quintessenz Verlags-GmbH OCI - Copyright Quintessenz Verlags-GmbH TA - J Adhes Dent JT - The Journal of Adhesive Dentistry IS - 1757-9988 (Electronic) IP - 4 VI - 17 PST - ppublish DP - 2015 PG - 347-352 LA - en TI - Effect of Matrix Metalloproteinase-inhibiting Solutions and Aging Methods on Dentin Bond Strength LID - 10.3290/j.jad.a34594 [doi] FAU - Perote, Letícia C. C. Costa AU - Perote L FAU - Kamozaki, Maria Beatriz Beber AU - Kamozaki M FAU - Gutierrez, Natália C. AU - Gutierrez N FAU - Tay, Franklin R. AU - Tay F FAU - Pucci, Cesar R. AU - Pucci C CN - OT - aging OT - matrix metalloproteinase OT - resin composite OT - tensile strength AB - Purpose: This study examined the effects of matrix metalloproteinase-inhibiting solutions and aging methods on the bond strength between resin composite and human dentin. Materials and Methods: Crown segments of 105 human non-carious molars were bonded using simulated pulpal pressure at 20 cm water pressure. The teeth were randomly split into 5 groups according to the solution applied: CG (control, no solution), CHX (0.2% chlorhexidine), EPE (10% ethanolic propolis extract), APE (aqueous propolis extract), and E (70% ethanol). Each solution was left on the acid-etched dentin for 1 min. Adper Single Bond 2 and resin composite (Filtek Z350 XT) were applied to all specimens. The 5 groups were subdivided according to the aging method: SI (sectioned immediately); S (storage in artificial saliva for 6 months); and T (thermomechanical aging with 240,000 mechanical cycles and 1000 thermal cycles). Specimens were sectioned into sticks and subjected to microtensile testing. Bond strength data were analyzed by two-factor ANOVA followed by a post-hoc Tukey's test (α = 0.05). Results: For the factor "solution", there was no significant difference among the groups (p = 0.32). For the factor "aging method", significant differences were found (p < 0.001), with the following mean bond strengths (MPa): SI: 31.1; S: 24.4; T: 26.8. Conclusions: The use of matrix metalloproteinase-inhibiting solutions on dentin as an adjunct to the application of an etch-and-rinse adhesive does not prevent the loss of bond strength after aging. Nevertheless, these solutions have no adverse effect on adhesion to tooth structure. AID - 843229