Quintessence International, 5/2020
DOI: 10.3290/j.qi.a44392, PubMed-ID: 32328595Seiten: 418-429, Sprache: Englisch
Objectives: This paper is aimed at addressing the urgent need to develop a protocol that will address the operatory and clinical aspects of dental care during the Coronavirus disease 2019 (COVID-19) outbreak.
Data sources: The epidemiology, clinical signs and symptoms, and modes of transmission of COVID-19 are presented. This protocol was established as an international collaboration of three dental universities: Hadassah School of Dental Medicine, Israel; University of Rochester Medical Center, USA; and the University of Pennsylvania, USA. This protocol is based on a detailed review of the existing English language literature as well on the logistic and clinical experience of each facility and the opinion of the authors. The protocol is designed for a hospital setting and includes considerations related to dental treatment in both healthy subjects and those suspected or diagnosed with COVID-19. The first part of this review discusses operatory considerations; the second part discusses general dental clinical aspects; the third part discusses endodontic considerations; and the fourth part discusses surgical aspects. This protocol may be applicable to other future similar pandemics.
Conclusion: Logistic and clinical steps are required to provide dental care during the COVID-19 outbreak while preventing cross-contamination and protecting the dental team during the provision of care.
Schlagwörter: coronavirus, COVID-19, dental, endodontics, infection control, pulpitis
Chinese Journal of Dental Research, 2/2012
PubMed-ID: 23509836Seiten: 145-152, Sprache: Englisch
Objective: To observe the effects of ionic dissolution products on nano-sized 58S bioactive glass (nano-58S) on proliferation and specific osteogenic genes expression in MG-63 cells.
Methods: Ionic dissolution products were prepared by incubating nano-58S or sol-gel bioactive glass 58S (58S) particulates in Dulbecco's modified Eagle's medium (DMEM) at 1% w/v for 24 hr and filtrated through 0.22 µm filters to remove the particulates. MG-63 cells were cultured in the nano-58S extraction, 58S extraction, and DMEM, respectively, for different time periods to assay the proliferation, mRNA expression of alkaline phosphatase (ALP), Cbfa1, Collagen type I (Col-I) and osteocalcin (OCN), as well as ALP staining, activity and matrix mineralisation.
Results: In the nano-58S group, cell proliferation and mRNA expression of ALP, Cbfa1 and OCN were significantly enhanced in a time-dependent manner compared with the control group. mRNA expression of Cbfa1 on day 4 and OCN on day 7 was significantly higher than that in the 58S group. Moreover, there was significantly more ALP protein expression and mineralisation in the nano-58S group than in the 58S group.
Conclusion: The nano-58S enhanced proliferation, osteogenic markers expression in MG-63 cells and induced stronger mineralization than 58S.
Schlagwörter: human osteoblast-like cells, nano-sized bioactive glass, gene expression, bone, osteogenesis