PMID- 36479892 OWN - Quintessenz Verlags-GmbH CI - Copyright Quintessenz Verlags-GmbH OCI - Copyright Quintessenz Verlags-GmbH TA - Chin J Dent Res JT - Chinese Journal of Dental Research IS - 1867-5646 (Electronic) IS - 1462-6446 (Print) IP - 4 VI - 25 PST - ppublish DP - 2022 PG - 269-275 LA - en TI - MiR-146a-5p Promotes Dental Stem Cells Osteo/odontogenic Differentiation through NF-Kappa B Signaling Pathway by Targeting TRAF6 LID - 10.3290/j.cjdr.b3628171 [doi] FAU - Yu, Xin AU - Yu X FAU - Lu, Jian Feng AU - Lu J FAU - Gao, Mei Qin AU - Gao M FAU - Xiong, Bin AU - Xiong B FAU - Xia, Wen Qian AU - Xia W CN - OT - dental stem cells OT - miR-146a-5p OT - NF-Kappa B signalling pathway OT - osteo/odontogenic differentiation OT - TRAF6 AB - Objective: To screen miRNAs that could simultaneously regulate osteo/odontogenic differentiation of multiple stem cells, including dental pulp stem cells (DPSCs), stem cells from the apical papilla (SCAPs) and periodontal ligament stem cells (PDLSCs). Methods: Differentially expressed miRNAs analysis on three miRNA microarrays data of dental stem cells undergoing osteo/odontogenic differentiation (GSE138180, GSE154466 and GSE159508) was performed, and miR-146a-5p were identified by bioinformatic prediction, dual-luciferase reporter assay and quantitative real-time polymerase chain reaction (PCR). In addition, differentially expressed genes between miR-146a-5p overexpressed group and control group (GSE79341) were applied for KEGG pathways enrichment analysis. Results: MiR-146a-5p expression increased in the osteo/odontogenic differentiation of DPSCs, SCAPs and PDLSCs. Tumour necrosis factor receptor (TNFR)-associated factor 6 (TRAF6) was identified as the target gene of miR-146a-5p. Furthermore, miR-146a-5p could influence the NF-Kappa B signalling pathway. Conclusion: This study suggests that miR-146a-5p could promote differentiation in multiple dental stem cells through the NF-Kappa B signalling pathway by targeting TRAF6. AID - 3628171