PMID- 36479894 OWN - Quintessenz Verlags-GmbH CI - Copyright Quintessenz Verlags-GmbH OCI - Copyright Quintessenz Verlags-GmbH TA - Chin J Dent Res JT - Chinese Journal of Dental Research IS - 1867-5646 (Electronic) IS - 1462-6446 (Print) IP - 4 VI - 25 PST - ppublish DP - 2022 PG - 285-291 LA - en TI - Expressions of GLUT-1, PK-M2 and HIF-1α and Mutation Status of BRAF in Odontogenic Keratocysts LID - 10.3290/j.cjdr.b3628195 [doi] FAU - You, Zhu AU - You Z FAU - Du, Jing AU - Du J FAU - Xu, Li Li AU - Xu L FAU - Zhang, He Yu AU - Zhang H FAU - Li, Xue Fen AU - Li X FAU - Sun, Zhi Peng AU - Sun Z FAU - Sun, Li Sha AU - Sun L CN - OT - BRAF gene OT - GLUT-1 OT - HIF-1α OT - odontogenic keratocyst OT - PK-M2 AB - Objective: To investigate the expressions and clinicopathological features of glucose transporter 1 (GLUT-1), pyruvate kinase M2 (PK-M2) and hypoxia-inducible factor 1α (HIF-1α) in odontogenic keratocysts (OKCs), and to investigate the mutation status of v-raf murine sarcoma viral oncogene homolog B1 (BRAF). Methods: Following a retrospective review of the clinicopathological data of 28 OKC cases, the expressions of GLUT-1, PK-M2 and HIF-1α in these tissue samples were detected through immunohistochemistry. The BRAF mutation statuses of all cases were examined using polymerase chain reaction amplification and direct sequencing. Results: The expression levels of HIF-1α varied in 96.4% of OKC tissues, and there were higher positive rates of PKM2 (100%) and GLUT-1 (100%) in these tissues. None of the 28 OKC samples carried the BRAF mutation. Conclusion: The positive expressions of GLUT-1, PK-M2 and HIF-1α indicate that patients with OKCs undergo anaerobic glycolysis to a certain extent, but these processes appear to be irrelevant to clinicopathological features and to the BRAF mutation. AID - 3628195