PMID- 29335687 OWN - Quintessenz Verlags-GmbH CI - Copyright Quintessenz Verlags-GmbH OCI - Copyright Quintessenz Verlags-GmbH TA - Oral Health Prev Dent JT - Oral Health and Preventive Dentistry IS - 1757-9996 (Electronic) IP - 4 VI - 16 PST - ppublish DP - 2018 PG - 369-373 LA - en TI - Structure of the Bacterial Community in Different Stages of Early Childhood Caries LID - 10.3290/j.ohpd.a39691 [doi] FAU - Ximenes, Marcos AU - Ximenes M FAU - Armas, Rafael Dutra de AU - Armas R FAU - Triches, Thaisa Cezária AU - Triches T FAU - Cardoso, Mariane AU - Cardoso M FAU - Vieira, Ricardo de Souza AU - Vieira R CN - OT - 16S rRNA gene OT - deciduous OT - dental caries OT - tooth AB - Purpose: To characterise in vivo the structure of bacterial communities in decayed and sound primary teeth. Materials and Methods: Samples of biofilms were collected from three groups of patients with complete and exclusively primary dentition (n = 45): G1: sound teeth (n = 15); G2: enamel lesion (n = 15); G3: dentin lesion (n = 15). DNA was extracted (CTAB 2%) from the biofilm, the partial 16S rRNA gene was amplified with Bacteria Universal Primers (BA338fGC - UN518r) and subjected to DGGE (denaturing gradient gel electrophoresis). Multidimensional scaling and ANOSIM (analysis of similarity) were employed to determine the structure of the bacterial communities. The amplicon richness was determined by averaging amplicons, with the differences between treatments determined with ANOVA, while means were compared using Tukey's test (p < 0.05). Results: Compared to sound teeth, a greater variety of bacterial communities was found in decayed teeth. Despite the differences between the bacterial communities of sound teeth and decayed teeth, the Venn diagram showed that the samples had 38 amplicons in common. Greater amplicon richness was observed in samples of decayed teeth (enamel: 20.5 ± 2.7; dentin: 20.1 ± 2.8) compared with the sound samples (12.0 ± 4.3) (p AID - 842160