PMID- 36416604 OWN - Quintessence Publishing Company, Ltd. CI - Copyright Quintessence Publishing Company, Ltd. OCI - Copyright Quintessence Publishing Company, Ltd. TA - Oral Health Prev Dent JT - Oral Health and Preventive Dentistry IS - 1757-9996 (Electronic) IP - 1 VI - 20 PST - epublish DP - 2022 PG - 465-474 LA - en TI - Salivary Flow Rate During Toothbrushing LID - 10.3290/j.ohpd.b3601691 [doi] FAU - Pulfer, Anina M. AU - Pulfer A FAU - Attin, Thomas AU - Attin T FAU - Wegehaupt, Florian J. AU - Wegehaupt F CN - OT - hydrogen ion concentration OT - saliva OT - salivary flow rate OT - toothbrushing OT - toothpastes AB - Purpose: To determine the salivary flow rate and subsequent dilution of toothpaste and assess the pH of oral fluids during toothbrushing with toothpastes of various pHs. Materials and Methods: The study was conducted as an in-vivo trial involving 30 healthy volunteers. The participants took part in a series of trials distributed over four appointments. After a screening check, in which the participants’ stimulated and unstimulated salivary flow rate and buffering capacities were determined, four test series involving toothbrushing were conducted. Participants brushed their teeth using a manual toothbrush for 2 min: once without toothpaste and three times using toothpastes of varying pHs. The salivary flow rate and subsequent dilution of the toothpaste was determined. Additionally, the pH of the collected oral fluid was analysed. Results: Brushing teeth with toothpaste caused a statistically significant increase in salivary flow rate (median/IQR in ml/min) (Elmex Kariesschutz 3.29/1.36, Colgate Total Original 3.23/1.08, Elmex Sensitive Professional 3.18/1.39) when compared to brushing teeth using a manual toothbrush without toothpaste (1.85/0.78) (p < 0.05). The variation in pH of the oral fluid samples was dictated primarily by the pH of the toothpaste used. Conclusion: The salivary flow rate when brushing using toothpaste was similar across all tested toothpastes, independent of pH, and had an average median of 3.23 ml/min. The dilution of 1 g of toothpaste during a standard toothbrushing procedure of 2 min is therefore approximately at a ratio of one part toothpaste to 6.5 parts saliva. AID - 3601691