In this study, the primary culture of bovine mandibular osteoblast cells in a microculture assay has been used to further investigate the interaction of mineralizing osteoblast cultures with implant surfaces by using correlative microscopic techniques. Rapid differentiation and mineralization of osteoblast cultures grown on titanium alloy surfaces was observed. The successful short-term culture of mineralizing mandibular osteoblasts on titanium alloy surfaces occurred without the formation of a tenacious adhesive interface between the alloplastic material and the multilayered cell culture. Keywords: cell culture, dental implant, interface, osteoblast, titanium alloy