Objective: To histologically evaluate the effects of a novel human recombinant amelogenin (rAmelX) on periodontal wound healing/regeneration in intrabony defects. Method and materials: Intrabony defects were surgically created in the mandible of three minipigs. Twelve defects were randomly treated with either rAmelX and carrier (test group) or with the carrier only (control group). At 3 months following reconstructive surgery, the animals were euthanized, and the tissues histologically processed. Thereafter, descriptive histology, histometry, and statistical analyses were performed. Results: Postoperative clinical healing was uneventful. At the defect level, no adverse reactions (eg, suppuration, abscess formation, unusual inflammatory reaction) were observed with a good biocompatibility of the tested products. The test group yielded higher values for new cementum formation (4.81 ± 1.17 mm) compared to the control group (4.39 ± 1.71 mm) without reaching statistical significance (P = .937). Moreover, regrowth of new bone was greater in the test compared to the control group (3.51 mm and 2.97 mm, respectively, P = .309). Conclusions: The present results provided for the first-time histologic evidence for periodontal regeneration following the use of rAmelX in intrabony defects, thus pointing to the potential of this novel recombinant amelogenin as a possible alternative to regenerative materials from animal origins. Schlagwörter: amelogenin, enamel matrix derivative, intrabony defects, periodontal regeneration, recombinant, wound healing

Purpose: To investigate and compare the production tolerances of six different commercially available implant scan bodies (SBs), with the null hypothesis that there would be no tolerances or significant differences between the different SBs. Materials and Methods: Six different implant SBs (IO 6A-B and IO 2B-B, Nobel Biocare; RC 4.1 mm 025.4915 and RN 4.8 mm 048.168, Straumann; KR 352KR1A0, BTK Dental Implants; and AANISR4013T, MegaGen) were evaluated. Five specimens of each SB type (total N = 30 samples) were screwed onto the corresponding implant analogs and underwent dimensional analysis with optical microscopy (QVI Smartscope Flash 200, Optical Gaging Products) and precision probing (R 0.25, Renishaw). The outcome variables were SB height, diameter, and angle of the flat face on the top (plane). All measurements were compared to the corresponding computer-assisted design library measurements as a reference to assess the tolerances. Statistical analyses were performed to compare the results obtained with the different SBs. Results: Tolerances in the manufacturing of the SBs were reported in height, diameter, and plane measurements, and statistically significant differences between the different types of SBs were found. Therefore, the null hypothesis was rejected. Most of the deviations and tolerances were reported in height measurements with conical connection implants. Conclusions: Production tolerances and statistically significant differences were found among the six commercially available SBs evaluated in this study. Additional studies with larger sample sizes and other types of SBs are needed.

Seit der Einführung der Socket-Shield-Technik durch Hürzeler und Mitarbeiter sind 10 Jahre vergangen. Seitdem hat sich viel weiterentwickelt bei dieser partiellen Extraktionstherapie, bei der die eigene Zahnwurzel des Patienten zum Erhalt des Parodonts und periimplantären Gewebes eingesetzt wird. Im vorliegenden Artikel werden Spezifikationen, Arbeitsschritte, Instrumente und Verfahren diskutiert, die das Ergebnis umfangreicher Erfahrungen bei der Verfeinerung der Socket-Shield-Technik sind, wie wir sie heute kennen. Ein zuverlässiges und reproduzierbares Protokoll ist eine zwingende Voraussetzung für den Ersatz eines Zahns in der ästhetischen Zahnmedizin. Zudem hilft das standardisierte Protokoll dabei, prozedural konsistente Daten zu dieser Technik zu publizieren. Erläutert wird ein reproduzierbares Schritt-für-Schritt-Protokoll für die Socket-Shield-Technik bei einer Sofortimplantation mit Sofortprovisorium als Ersatz für einwurzelige Zähne.

Ten years have passed since Hürzeler and coworkers first introduced the socket-shield technique. Much has developed and evolved with regard to partial extraction therapy, a collective concept of utilizing the patient's own tooth root to preserve the periodontium and peri-implant tissue. The specifications, steps, instrumentation, and procedures discussed in this article are the result of extensive experience in refining the socket-shield technique as we know it today. A repeatable, predictable protocol is requisite to providing tooth replacement in esthetic dentistry. Moreover, a standardized protocol provides a better framework for clinicians to report data relating to the technique with procedural consistency. This article aims to illustrate a reproducible, step-by-step protocol for the socket-shield technique at immediate implant placement and provisionalization for single-rooted teeth.

Predictable and effective surgical techniques that aim to increase the width of keratinized gingiva, relocate the mucogingival junction, and deepen the vestibule often involve soft tissue autografts; however, soft tissue autograft supply is limited and its harvesting is associated with patient morbidity. With a strip autograft and xenogeneic collagen matrix (XCM) technique combination, autograft harvest requirements and patient morbidity are reduced. In this histologic evaluation, 12 strip autograft/XCM biopsy samples were compared with 3 reference samples of palatal strip autografts. Tissue morphology, keratin, and collagen expression appear identical, indicating that the combined grafting technique provides desired and physiologically normal keratinized gingiva.

Large areas of mucogingival alterations may result from advanced regenerative procedures. This prospective case series study was performed to introduce and evaluate a surgical approach that combines the strip gingival graft technique with the use of a xenogeneic collagen matrix. The primary outcome measurement was the increase in keratinized tissue width from baseline to 12 months postprocedure. Twenty patients were enrolled, and they all completed the 12-month evaluation. All treated sites exhibited a significant gain in keratinized tissue at 12 months, with a mean width of 6.33 mm (SD: 2.16), while there was a 43% contraction of the grafted area at 6 months. Tissue dimensions remained stable between 6 and 12 months. The use of the combination graft was well accepted by the patients, with minimal morbidity according to the patients' low self-reported pain and the low utilization of pain medication.

Ziel: Diese prospektive Fallserie evaluierte die Verwendung einer neuen nichtresorbierbaren titanverstärkten Membran (hochdichtes Polytetrafluorethylen) in Kombination mit einer Mischung aus anorganischem Knochenmineral boviner Herkunft (anorganic bovine bone-derived mineral, ABBM) und partikuliertem autogenem Knochen für die vertikale Augmentation von defizienten Alveolarkämmen. Material und Methode: Die vertikale Kammaugmentation erfolgte mit einer Mischung aus ABBM und partikuliertem autogenem Knochen, die mit einer neuen nichtresorbierbaren titanverstärkten Membran abgedeckt wurde. Vor und nach dem Eingriff wurden Messungen der Kammhöhe durchgeführt; Komplikationen wurden aufgezeichnet; zur histologischen Bewertung wurden Biopsieproben entnommen. Ergebnisse: An 19 Patienten wurden 20 vertikale Kammaugmentationen durchgeführt. In allen behandelten Defekten zeigte sich eine hervorragende Knochenbildung mit einem durchschnittlichen Knochengewinn von 5,45 mm (Standardabweichung: 1,93 mm). Die Heilungsphase verlief ereignislos; Komplikationen wurden nicht beobachtet. 8 Proben wurden histologisch untersucht: Im Durchschnitt bestanden die Proben zu 36,6 % aus autogenem oder regeneriertem Knochen, zu 16,6 % aus ABBM und zu 46,8 % aus Markräumen. An den Proben waren weder Entzündungs- noch Fremdkörperreaktionen zu beobachten. Schlussfolgerung: Die Behandlung vertikaler Alveolarkammdefekte mittels gesteuerter Knochenregeneration unter Verwendung einer Mischung aus autogenem Knochen und ABBM sowie einer neuen nichtresorbierbaren titanverstärkten Membran darf als erfolgreich gelten. Schlagwörter: bovines anorganisches Knochenmineral, Fallserie, gesteuerte Knochenregeneration, nichtresorbierbare Membran, vertikale Augmentation

Purpose: This prospective case series evaluated the use of a new titanium-reinforced nonresorbable membrane (high-density polytetrafluoroethylene), in combination with a mixture of anorganic bovine bone-derived mineral (ABBM) and autogenous particulated bone, for vertical augmentation of deficient alveolar ridges. Materials and Methods: A mixture of ABBM and autogenous particulated bone was used for vertical ridge augmentation and covered with a new titanium-reinforced nonresorbable membrane. Ridge measurements were obtained before and after the procedure, complications were recorded, and biopsy specimens were taken for histologic examination. Results: Twenty vertical ridge augmentation procedures were carried out in 19 patients. All treated defect sites exhibited excellent bone formation, with an average bone gain of 5.45 mm (standard deviation 1.93 mm). The healing period was uneventful, and no complications were observed. Eight specimens were examined histologically; on average, autogenous or regenerated bone represented 36.6% of the specimens, ABBM 16.6%, and marrow space 46.8%. No inflammatory responses or foreignbody reactions were noted in the specimens. Conclusion: The treatment of vertically deficient alveolar ridges with guided bone regeneration using a mixture of autogenous bone and ABBM and a new titanium-reinforced nonresorbable membrane can be considered successful. Schlagwörter: anorganic bovine bone-derived mineral, case series, guided bone regeneration, nonresorbable membrane, vertical augmentation

This clinical case report describes and demonstrates the successful use of purified recombinant human platelet-derived growth factor in conjunction with autogenous bone, anorganic bovine bone-derived mineral, and a barrier membrane to reconstruct severe alveolar bone defects. Significant horizontal bone regeneration was achieved in the posterior maxillary region. Three implants were placed into the newly formed ridge and demonstrated stable crestal bone after 36 months of loading.